polyclonalantibody相关论文
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A novel gene encoding a 34 kDa protein of Babesia orientalis(designated BoP34)was obtained by immunoscreening of a cDNA ......
Objectives: ADP-Glc pyrophosphorylase(AGPase)is a key rate-limiting enzyme in maize starch biosynthesis,which exist in a......
Cyprinid herpesvirus II(CyHV-2)is the main pathogen causing herpesviral haematopoietic necrosis disease in Carassius aur......
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We previously demonstrated that serine protease inhibitor BmSPI could strongly inhibit subtilisin A.In order to characte......
A novel real-time immuno-PCR (RT-IPCR) assay for the determination of 3,4-dichlorinated biphenyl (IUPAC PCB12) is de......
Objectives: To detect the secretion and localization of Toxoplasma gondii Rhoptry Protein 16(ROP16)during invasion o......
Small heat shock protein (sHSP) is a family of proteins induced by heat stress. We obtained a sequence from the cDNA......
采用RT-PCR方法分段扩增PB2基因,将目的基因定向克隆至含HisTAG的原核表达载体pET-32a,经序列验证正确后,转化BL21大肠杆菌,诱导表......
在沙门菌检测过程中,奇异变形杆菌经常作为一种假阳性菌而出现。为提高沙门菌分离鉴定的准确性,我们制备了奇异变形杆菌的多克隆抗......
采用新的试验方法制备出纯度较高的抗猪Sn的鼠纯化IgG,并证实此种抗体可以阻碍PRRSV感染PAM,为研究猪Sn各结构域的作用提供参考,为......
目的制备兔抗人STEAP4多克隆抗体及其特性鉴定。方法应用DNAStar软件对STEAP4的蛋白序列进行分析,选择STEAP4基因编码蛋白的近N端......
In vitro differentiation of human adipose-derived adult stromal cells into neuron-like cells in hipp
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目的 在大肠杆菌中克隆表达和纯化寨卡病毒(Zika Virus,ZIKV)包膜糖蛋白(Envelope Protein,E)及第三结构域(Envelope DomainⅢ,ED......
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目的 构建谷胱甘肽-S-转移酶(GST)和中期因子(MK)融合蛋白的原核表达质粒,并表达和纯化蛋白,制备多克隆抗体.方法 通过RT-PCR技术......
目的:制备CD151-整合素α6β1结合域的多克隆抗体,探讨其免疫学特性.方法:化学合成CD151-整合素α6β1结合域的多肽CGQRDHASNIYKVE......
Expression of toll-like receptor 4 and MD-2 gene and protein in Kupffer cells after ischemia-reperfu
AIM: To investigate the expression of toll-like receptor 4(TLR4) and MD-2 gene and protein in Kupffer cells (KCs)and the......
Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B fromEc
ABSTRACT Objective:To immunize rabbits with12 and16 kDa recombinant subunits of antigenB from Echinococcus granulosus (E......
Development of a Polyclonal Antibody-based AC-ELISA and Its Comparison with PCR for Diagnosis of Can
A polyclonal antibody-based antigen-capture ELISA (AC-ELISA) has been developed for detection of Canine parvovirus (CPV)......
目的 分析上海HIV-1分离株病毒感染因子(Vif)蛋白编码基因突变特点,构建HIV-1vif基因原核表达质粒,并了解其免疫原性.方法 采用RT-......
目的获得人 Fas L蛋白 ,并对其功能进行初步研究。方法用 DNA重组法构建了 Fas L c DNA和谷胱甘肽转硫酶融合原核表达质粒 p GEX- ......
